1. The Extended Family of Protein Tyrosine Phosphatases
Andrés Alonso, Caroline E. Nunes-Xavier, Yolanda Bayón, and Rafael Pulido
2. Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome
Caroline E. Nunes-Xavier and Rafael Pulido
3. Expression, Purification, and Kinetic Analysis of PTP Domains
Mihaela Mentel, Rodica A. Badea, Georgiana Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, and Stefan E. Szedlacsek
4. Peptide Microarrays for Real Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples
Liesbeth Hovestad-Bijl, Jeroen van Ameijde, Dirk Pijnenburg, Riet Hilhorst, Rob Liskamp, and Rob Ruijtenbeek
5. Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B
Sandra Luna, Janire Mingo, Olaia Aurtenetxe, Lorena Blanco, Laura Amo, Jan Schepens, Wiljan J. Hendriks, and Rafael Pulido
6. Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates
Laura Spinelli and Nicholas R. Leslie
7. Assessing the Biological Activity of the Glucan Phosphatase Laforin
Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, and Pascual Sanz
8. Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents
Yunpeng Bai, Zhi-Hong Yu, and Zhong-Yin Zhang
9. Analyzing Pseudophosphatase Function
Shantá D. Hinton
10. Crystallization of PTP Domains
Colin Levy, James Adams, and Lydia Tabernero
11. NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases
Wolfgang Peti and Rebecca Page
12. Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs)
Christopher J. Caunt, Andrew M. Kidger, and Stephen M. Keyse 13. In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions
Sina Koch, Irene Helbing, Sylvia-Annette Böhmer, Makoto Hayashi, Lena Claesson-Welsh, Ola Söderberg, and Frank-D. Böhmer
14. Use of Dominant Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates
Vegesna Radha
15. Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains
Andrew William Stoker 16. Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling
Eynat Finkelshtein, Einat Levy-Apter, and Ari Elson
17. Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Haemostasis
Souad Rahmouni, Alexandre Hego, Céline Delierneux, Odile Wéra, Lucia Musumeci, Lutz Tautz, and Cécile Oury
18. Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis
Mathieu Amand, Charlotte Erpicum, Christine Gilles, Agnès Noël, and Souad Rahmouni
19. Studying Protein-Tyrosine Phosphatases in Zebrafish
Alexander James Hale and Jeroen den Hertog
20. Live staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands
Namrata Bali, Hyung-Kook (Peter) Lee, and Kai Zinn
21. Methods to Study Protein Tyrosine Phosphatases Acting on Yeast MAPKs
Almudena Sacristán-Reviriego, María Molina, and Humberto Martín
