1. An Introduction to P-type ATPase research

Poul Nissen

Part I: Protein Production, Isolation, Purification, and Stabilization

2. Purification of Na, K-ATPase from Pig Kidney

Natalya U. Fedosova

3. Preparation of Ca²⁺-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum

Jesper V. Møller and Claus Olesen

4. Isolation of H⁺/K⁺-ATPase-enriched Membrane Fraction from Pig Stomachs

Kazuhiro Abe and Claus Olesen

5. Overproduction of PiB-type ATPases

Xiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon

6. Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex

Hassina Azouaoui, Cédric Montigny, Aurore Jacquot, Raphaëlle Barry, Philippe Champeil, and Guillaume Lenoir

7. The Plasma Membrane Ca²⁺-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein

Verena Niggli and Ernesto Carafoli

8. Expression of Na, K-ATPase and H, K-ATPase Isoforms With The Baculovirus Expression System

Jan B. Koenderink and Herman G.P. Swarts

9. Time-dependent Protein Thermostability Assay

Ilse Vandecaetsbeek and Peter Vangheluwe

Part II: Activity Assays

10. Colorimetric Assays of Na, K-ATPase

Kathleen J. Sweadner

11. ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay Pankaj Sehgal, Claus Olesen, and Jesper V. Møller

12. Antimony-Phosphomolybdate ATPase Assay

Gianluca Bartolommei and Francesco Tadini-Buoninsegni

13. ATPase Activity Measurements using radiolabeled ATP

Herman G.P. Swarts and Jan B. Koenderink

14. Assaying P-type ATPases Reconstituted in Liposomes

Hans-Jürgen Apell and Bojana Damnjanovic

15. Coupling ratio for Ca²⁺ Transport by Calcium Oxalate Precipitation

Pankaj Sehgal, Claus Olesen, and Jesper V. Møller

16. Calcium Uptake in Crude Tissue Preparation

Philip A. Bidwell and Evangelia G. Kranias

17. Measuring H⁺ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems

18. Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives Magdalena Marek and Thomas Günther Pomorski

Part III: In vitro Functional Studies

19. The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases

Stefania J. Danko and Hiroshi Suzuki

20. Phosphorylation/Dephosphorylation Assays

Hiroshi Suzuki

21. Tryptophan Fluorescence Changes Related to Ca²⁺-ATPase Function

Pankaj Sehgal, Claus Olesen, and Jesper V. Møller

Part IV: Ligand Binding Studies

22. Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca²⁺-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling

Johannes D. Clausen, David B. McIntosh, David G. Woolley, and Jens Peter Andersen

23. Ca²⁺ Binding and Transport Studied with Ca²⁺/EGTA Buffers and ⁴⁵Ca²⁺

Pankaj Sehgal, Claus Olesen, and Jesper V. Møller

24. Assay of Copper Transfer and Binding to P_1B-ATPases

Teresita Padilla-Benavides and José M. Argüello