Preface...

Table of Contents...

Contributing Authors...

1. Analysis and Quantification of GPCR Allosteric Receptor-Receptor Interactions using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example
Dasiel O. Borroto-Escuela, Miguel Perez de la Mora, Michele Zoli, Fabio Benfenati, Manuel Narvaez, Alicia Rivera, Zaida Díaz-Cabiale, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Malgorzata Filip, Fang Liu, Rafael Franco, Luigi F. Agnati, and Kjell Fuxe

2. Analysis and Quantification of GPCR Heteroreceptor Complexes and their Allosteric Recpetor-Receptor Interactions using Radioligand Binding Autoradiography
Manuel Narvaez, Fidel Corrales, Ismel Brito, Ismael Valladolid-Acebes, Kjell Fuxe, and Dasiel O. Borroto-Escuela

3. On the Study of D4R-Mor Receptor-Receptor Interactions in the Rat Caudate Putamen: Relevance on Morphine Addiction
Alicia Rivera, Alejandra Valderrama-Carvajal, Diana Suárez-Boomgaard, Kirill Shumilov, M. Ángeles Real, Kjell Fuxe, and Belén Gago

4. Use of Superfused Synaptosomes to Understand the Role of Receptor-Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region
Sarah Beggiato, Sergio Tanganelli, Tiziana Antonelli, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela, and Luca Ferraro

5. Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptor (mAChRs) in Primary Neuronal Hippocampal Cultures through use of Biochemical and Morphological Approaches
Valentina Di Liberto, Giuseppa Mudo, Dasiel O. Borroto-Escuela, Kjell Fuxe, and Natale Belluardo

6. Electrophysiological Approach to GPCR-RTK Interaction Study in Hippocampus of Adult Rats
Davide Lattanzi, David Savelli, Michael Di Palma, Stefano Sartini, Silvia Eusebi, Dasiel O. Borroto-Escuela, Riccardo Cuppini, Kjell Fuxe, and Patrizia Ambrogini

7. In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor-Receptor Interactions to the Central Nervous System Signaling
Sergio Tanganelli, Tiziana Antanelli, Sarah Beggiato, Maria Cristina Tomasini, Kjell Fuxe, Dasiel O. Borroto-Escuela, And Luca Ferraro

8. Behavioral Methods to Study the Impact of Receptor-Recepetor Interactions in Fear and Anxiety
Miguel Pérez de la Mora, José del Carmen Rejón-Orantes, Minerva Crespo-Ramírez, Dasiel O. Borroto-Escuela, and Kjell Fuxe

9. Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior
Antonio Flores-Burgess, Carmelo Millón, Belen Gago, José Angel Narváez, Kjell Fux, and Zaida Díaz-Cabiale

10. Double Fluorescent Knock-In-Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution
Lyes Derouiche, Stéphane Ory, and Dominique Massotte

11. Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays
Ping Su, Frankie H.F. Lee, and Fang Liu

12. Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors, or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System
Irene Reyes-Resina, Eva Martínez-Pinilla, Dasiel O. Borroto-Escuela, Kjell Fuxe, Gemma Navarro, and Rafael Franco

13. Biomolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells
Doungkamol Alongkronrusmee, Val. J. Watts, and Richard M. van Rijn

14. Detection and Quantitative Analysis of Dynamic GPCRs Interactions using Flow Cytometry-Based FRET
Barbara Chruścicka, Shauna E. Wallace Fitzsimons, Clémentine M