1. Transient Gene Expression in Suspension HEK293-EBNA1 Cells
Denis L'Abbé, Louis Bisson, Christian Gervais, Eric Grazzini and Yves Durocher
2. Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells
Joanna Pieprzyk, Samuel Pazicky and Christian Löw
3. PEI-Mediated Transient Gene Expression in CHO Cells
Yashas Rajendra
4. Stable Expression by Lentiviral Transduction of Cells
Natascha Gödecke, Hansjörg Hauser and Dagmar Wirth
5. Inducible Protein Production in 293 Cells using the piggyBac Transposon System
Iacovos P. Michael and Andras Nagy
6. Recombinant CHO Cell Pool Generation using piggyBac Transposon System
Sowmya Balasubramanian
7. Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression
Cristina Parola, Derek Mason, Andreas Zingg, Daniel Neumeier and Sai T. Reddy
8. Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
David I. Fisher
9. CHO and HEK293 Cell Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors
David L. Hacker, Laurence Durrer and Soraya Quinche
10. Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary Cells in Suspension
Dominique Monteil and Jeffrey Kuan
11. Continuous and Integrated Expression and Purification of Recombinant Antibodies
Sebastian Vogg, Moritz Wolf and Massimo Morbidelli
12. High Throughput Transfection of HEK293 Cells for Transient Protein Production
Tia A. Arena, Peter D. Harms and Athena W. Wong
13. Microfluidic Transfection for High-Throughput Mammalian Protein Expression
Kristina Woodruff and Sebastian J. Maerkl
14. Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production
Sarah Inwood, Michael Betenbaugh, Madhu Lal and Joseph Shiloach
15. Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells
Kevin Kellner, Ankur Solanki, Thomas Amann, Nga T Lao and Niall Barron
16. Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells
Qiong Wang, Cheng-Yu Chung, Julian N. Rosenberg, Geng Yu and Michael J. Betenbaugh
17. Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)
Daniel Blessing, Nicole Déglon and Bernard L. Schneider
18. Considerations in the Use of Codon Optimization for Recombinant Protein Expression
Vincent P. Mauro and Stephen A. Chappell
19. Versatile Cell-free Protein Synthesis Systems based on Chinese Hamster Ovary Cells
Lena Thoring and Stefan Kubick
