Part I: Vectors and Transformation

1. Chloroplast Based Expression of Recombinant Proteins by Gateway(R) Cloning Technology

Johanna Gottschamel and Andreas Lössl

2. Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors

Ruifeng He

3. Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

Pooja Saxena, Eva C. Thuenemann, Frank Sainsbury, and George P. Lomonossoff

4. Transient Protein Expression by Agroinfiltration In Lettuce

Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa Mcnulty, Kahlin Leuzinger, Huafang Lai

5. Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii

Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena

Part II: Product Improvements

6. Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes

Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini

7. Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation

Alexandra Castilho

8. Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants

Stéphanie Robert, Philippe V. Jutras, Moustafa Khalf, Marc-André D'Aoust, Marie-Claire Goulet, Frank Sainsbury and Dominique Michaud

Part III: Extraction and Scale-Up

9. Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE(R) Tobacco Mosaic Virus Vector

Lauren Moore, Krystal Hamorsky, and Nobuyuki

10. Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots

Manish L. Raorane, Joan O. Narciso, and Ajay Kohli

11. Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In Transplastomic Plants

Sherwin Barretto, Franck Michoux, and Peter J. Nixon

12. Plant Cell-Based Recombinant Antibody Manufacturing with A 200 L Orbitally Shaken Disposable Bioreactor

Nicole Raven, Stefan Schillberg, and Stefan Rasche

13. Liquid-Liquid Phase Separation of Oil Bodies From Seeds

Cory L. Nykiforuk

14. Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture

Lauri J. Reuter, Andrew J. Conley, and Jussi J. Joensuu

Part IV: Analysis

15. Molecular Analyses of Transgenic Plants

Kurniawan Rudi Trijatmiko, Felichi Mae Arines, Norman Oliva, Inez Slamet-Loedin, and Ajay Kohli

16. Analysis of Recombinant Proteins In Transgenic Rice Seeds: Identity, Localization, Tolerance To Digestion, And Plant Stress Response

Yuhya Wakasa and Fumio Takaiwa

17. Real-Time PCR-Based Quantitation Method for The Genetically Modified Soybean Line GTS 40-3-2

Kazumi Kitta, Reona Takabatake, and Junichi Mano

18. A Standardized Lepidopteran Bioassay to Investigate the Bioactivity Of Insecticidal Proteins Produced In Transgenic Crops

Gerson Graser and Frederick S. Walters

Part V: Antibodies for Pathogen Resistance

19. Generating Transgenic Plants