Part I: Purification and Phenotypic Characterization of B Cell Subsets
1. Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells
M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti
2. Purification and Immune-Phenotyping of B-1 Cells from Body Cavities of Mice
Vanessa Yenson and Nicole Baumgarth
3. Purification and Immunophenotypic Characterization of Murine B10 B Cells
Chao Hong and Xiao-Ming Gao
4. Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions
Jean-David Bouaziz, Hélène Le Buanec, and Adèle de Masson
Part II: Mechanisms of Immune Suppression by B Cells
5. IL-10 Detection in Murine B Cells: Pros and Cons of the Different Techniques
Jun Deng, Xiang Lin, and Liwei Lu
6. TGF-β Detection and Measurement in Murine B Cells: Pros and Cons of the Different Techniques
Yoshiyuki Mishima, Shunji Ishihara, Jonathan J. Hansen, and Yoshikazu Kinoshita
7. Characterization and Activity of Fas Ligand Producing CD5+ B Cells
Steven K. Lundy and Matthew W. Klinker
Part III: Methods for the Ex Vivo Generation/Expansion of IL-10-Producing B Cells
8. Utilization of a Lentiviral System for the Generation of B Cells with Regulatory Properties
Elisabeth Calderón-Gómez and Simon Fillatreau
9. Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines
Aurélie Tormo, Jiusheng Deng, Edouard Al-Chami, Ziouani Sonia, and Moutih Rafei
10. Toll-Like Receptor Ligation for the Induction of Regulatory B Cells
Luciën E.P.M van der Vlugt, Simone Haeberlein, Wouter de Graaf, Tyrill E.D Martha, and Hermelijn H. Smits
11. The Generation of Regulatory B Cells by Helminth Parasites
Adnan Khan, Sylvie Amu, Sean P. Saunders, and Padraic G. Fallon
12. Use of Co-Cultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions
Francesca Mion, Federica Benvenuti, Carlo Pucillo, and Gaetano Vitale
Part IV: Study of Regulatory B Cells in Pathological Settings
13. Regulatory B Cells in Experimental Mouse Models of Arthritis
Elizabeth C. Rosser and Claudia Mauri
14. Regulatory B Cells in Mouse Models of Systemic Lupus Erythematosus (SLE)
Takashi Matsushita
15. Regulatory B Cells in Allergic Airways Disease and Asthma
Prabitha Natarajan, Linda A. Guernsey, and Craig M. Schramm
16. Regulatory B Cells in Mouse Models of Intestinal Inflammation
Atsushi Nishida, Cindy W. Lau, Emiko Mizoguchi, and Atsushi Mizoguchi
17. Regulatory B Cells in Experimental Autoimmune Encephalomyelitis (EAE)
Avijit Ray and Sreemanti Basu
18. Regulatory B Cells, Helminths, and Multiple Sclerosis
Jorge Correale and Tomas Rivero Equiza
19. Generation and Identification of Tumor-Evoked Regulatory B Cells
Arya Biragyn, Catalina Lee-Chang, and Monica Bodogai
