Part I: Molecular Genetics and Tools
1 Identification and Analysis of Genes Associated with Inherited Retinal Diseases
Mubeen Khan, Zeinab Fadaie, Stéphanie S. Cornelis, Frans P.M. Cremers, and Susanne Roosing
2 eQTL Analysis in Retinal Research
Felix Graßmann
3 Testing for known Retinal Degeneration Mutants in Mouse Strains Khalid Rashid, Katharina Dannhausen, and Thomas Langmann
4 CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo
Daniela Benati, Valeria Marigo, and Alessandra Recchia
5 Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis Yuchen Lin, Sarah Irmscher, and Christine Skerka
Part II: Cell/Tissue Culture Models
6 Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells Caroline Brandl
7 Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture Francesca Mazzoni, Yingyu Mao, and Silvia C. Finnemann
8 Porcine RPE/Choroidal Explant Cultures Alexa Klettner and Yoko Miura
9 The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells
Manuela Völkner, Thomas Kurth, and Mike O. Karl
10 Cell Death Analysis in Retinal Cultures
Sarah L Roche, Ana M Ruiz-Lopez, and Thomas G Cotter
11 Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-derived Macrophages in Retinal Disease
Nancy J Reyes, Rose Mathew, and Daniel R Saban
Part III: Animal Models
12 Light Damage Models of Retinal Degeneration Christian Grimm and Charlotte E. Reme
13 Induction and Readout of Oxygen-Iinduced Retinopathy
Raffael Liegl, Claudia Priglinger, and Andreas Ohlmann
14 Generation and Analysis of Xenopus laevis Models of Retinal Degeneration using CRISPR/Cas9 Joanna M. Feehan, Paloma Stanar, Beatrice M. Tam, Colette Chiu, and Orson L. Moritz
15 Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases
Holger Dill and Utz Fischer
16 Drosophila melanogaster - A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa
Malte Lehmann, Elisabeth Knust, and Sarita Hebbar
Part IV: Retinal Imaging
17 Retinal Fundus Imaging in Mouse Models of Retinal Diseases
Anne F. Alex, Maged Alnawaiseh, Peter Heiduschka, and Nicole Eter
18 Phenotyping of Mouse Models with OCT
G. Alex Ochakovski and M. Dominik Fischer
19 Cell-specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy
Laurie L. Molday, Christiana L. Cheng, and Robert S. Molday
20 Immuno-TEM/STEM in Retinal Research
Sanae Sakami and Krzysztof Palczewski
21 Noninvasive Two-photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium Grazyna Palczewska, Timothy S. Kern, and Krzysztof Palczewski
Part V: Th