Rat Hybridomas and Rat Monoclonal Antibodies (1990)
Home > Medicine & Health Science textbooks > Medicine: general issues > Medical equipment and techniques > Medical research > Rat Hybridomas and Rat Monoclonal Antibodies (1990)
Rat Hybridomas and Rat Monoclonal Antibodies (1990)

Rat Hybridomas and Rat Monoclonal Antibodies (1990)

|
     0     
5
4
3
2
1




International Edition


About the Book

The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.

Table of Contents:
Introduction Part I IMMUNOCYTOMAS in LOUVAIN (LOU) RATS. Chapter 1 Rat Immuoglobulins. Chapter 2 The LOUVAIN (LOU) Rats. Chapter 3 Rat Immunocytomas (IR). Part II PRODUCTION of RAT-RAT HYBRIDOMAS. Chapter 4 Fusion Cell Lines. Chapter 5 Immunization of Rats. Chapter 6.I Fusion Procedure. Chapter 6.II Rat Hybridoma Prodcution: Scanning electron Microscopy of a Fusion Experiment Chapter 7 Selective Growth of Hybrids. Chapter 8 Cloning. Chapter 9.I Screening of Monoclonal Antibodies Directed Against Soluble Antigens Chapter 9.II Screening of Hybrid Supernatants for Monoclonal Antibodies Against Cellular Antigens Chapter 10 Isotyping of Rat Monoclonal Antibodies. Chapter 11 Adaptation to In Vivo Culture. Freezing and Thawing. Chapter 12 Freezing and Thawing Part III PRODUCTION, PURIFICATION, FRAGMENTATION and LABELING of MONOCLONAL ANTIBODIES. Chapter 13 In Vitro Production of Rat Monoclonal Antibodies. Chapter 14 In Vivo Production of Rat Monoclonal Antibodies. Chapter 15 Purification of Rat Monoclonal Antibodies from Ascitic Fluid - Serum or Culture Supernatant. Chapter 16 Quality Control of Purified Antibodies. Chapter 17 Fragmentation of Rat Monoclonal Antibodies. Chapter 18 Labeling of Monoclonal Antibodies. Part IV EXAMPLES of PRODUCTION and CHARACTERIZATION of RAT-RAT HYBRIDOMAS AGAINST VARIOUS Ag. Chapter 19.I Rat Monoclonal Antibodies Against Murine Immunoglobulins. Chapter 19.II Allotype Specific Monoclonal Antibodies to Rat Kappa Light Chain. Chapter 19.III Application of Rat Hybridomas to Studies on Ocular Immunobiology. Chapter 19.IV Rat Monoclonal Antibodies Against Rabbit Immunoglobulin. Chapter 19.V Preparation of Anti-Human IgG Monoclonal Antibodies Using Rat x Rat Hybridoma System. Chapter 19.VI Rat Monoclonal Antibodies Against Human Immunoglobulin. Chapter 19.VII Production and Characterization of Rat Monoclonal Antibodies Directed Against Human Apolipoprotein B. Chapter 19.VIII Studies on Rat-Rat Hybridoma Technique and its Application to Obtain Rat Monoclonal Antibodies Anti-Horse-radish Peroxidase. Chapter 19.IX Rat-Rat and Rat-Mouse Hybridomas Secreting Rat Monoclonal Anti-Horse-radish Peroxidase. Chapter 19.X LOU Rat Monoclonal Antibodies Against Xenopus Vitellogenin. Chapter 19.XI Production and Characterization of Rat-Rat Hybridomas Against DNP-Hapten. Rat x Rat Hybridomas Against Cellular Antigens. Chapter 20.I Rat Monoclonal Antibodies Against Human Leukocyte Differentiation Antigens. Chapter 20.II Rat Monoclonal Antibodies Specific for T Lymphocytes. Chapter 21.I Rat Monoclonal Antibodies Against Streptococcus mutans and Bacteroides gingivalis. Chapter 21.II A Specific Rat Monoclonal Antibody Against Yersinia enterocolitica Serogroup 0:8. Chapter 21.III Production of Rat Hybridoma Directed Against Yersinia enterocolitica. Chapter 22 Rat Monoclonal Antibodies Against Viral Antigens. Chapter 23 Contribution of Rat Monoclonal Antibodies to the Study of Schistosoma mansoni Infections. Part V SPECIFIC USES and PROPERTIES of RAT MONOCLONAL ANTIBODIES. Chapter 24.I Mouse Immunoglobulin Isotyping. Chapter 24.II Humoral Immune Suppression of Mice by Monoclonal Rat Anti-Mouse µHeavy Chain Antibodies. Chapter 24.III Rat Monoclonal Antibodies Against Mouse IgE. Chapter 24.IV Purification of Mouse Monoclonal Antibodies by Immunoaffinity Chromatography. Chapter 24.V Screening of Mouse Monoclonal Antibodies. Chapter 24.VI Use of Rat Monoclonal Antibodies in Immunohistochemistry Investigations. Chapter 24.VII Use of Rat-Rat Monoclonal Antibodies Labeled with Fluorochrome. Chapter 25.I Use of Rat Monoclonal Anti-Rat Allotype Antibodies in Immunohistoperoxidase Technique and ELISA. Chapter 25.II Use of Anti-Rat Kappa Light Chain Allotype Monoclonal Antibodies for the Quantification of Rat Monoclonal Antibodies in Ascitic Fluid or Serum. Chapter 26 Extracorporeal Removal of Arteriogenic Particles by Use of Rat Monoclonal Antibody Coupled to Sepharose as Specific Immunosorbent. Chapter 27 Antibacteria Antigens, Especially Streptococcus mutans. Chapter 28 Mercury-Induced Autoimmunity Production of Monoclonal Autoantibodies. Chapter 29 Rat Monoclonal Antibodies to Plant Pathogens. Chapter 30.I Rat Monoclonal Antibodies for Complement-Mediated and Cell-Medicated Cytolysis. Chapter 30.II Rat Antibodies and K Cells. Chapter 31 Absence of Viral Particles in Rat Hybridomas - An Ultrastructural Study. Chapter 32 Role of CODATA-HDB in the Centralization and Distribution of Information Concerning the Rat Hybridomas. Chapter33 List of Monoclonal Antibodies.


Best Sellers


Product Details
  • ISBN-13: 9781138561649
  • Publisher: Taylor & Francis Ltd
  • Publisher Imprint: CRC Press
  • Height: 254 mm
  • No of Pages: 534
  • Width: 178 mm
  • ISBN-10: 1138561649
  • Publisher Date: 28 Jan 2019
  • Binding: Paperback
  • Language: English
  • Weight: 965 gr


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Rat Hybridomas and Rat Monoclonal Antibodies (1990)
Taylor & Francis Ltd -
Rat Hybridomas and Rat Monoclonal Antibodies (1990)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Rat Hybridomas and Rat Monoclonal Antibodies (1990)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!