Part I: Animal Models
1. Collagen-induced Arthritis Models
Miyoshi Maya and Shuang Liu
2. Human Xenograft Model
Shuang Liu
3. Long-term Constant Subcutaneous Drug Administration
Shuang Liu and Maya Miyoshi
4. Clinical Scoring of Disease Activity in Animal Models
Maya Miyoshi and Shuang Liu
5. Histological Analysis of Arthritic Joints
Takeshi Kiyoi
6. Preparation of Joint Extracts
Shuang Liu and Erika Takemasa
Part II: Therapeutic Approach 7. Production of Immunizing Antigen Proteoliposome Using Cell-free Protein Synthesis System
Wei Zhou and Hiroyuki Takeda
8. Re-construction of Protein/Liposome Complex Yasuyuki Suzuki
9. Production of Neutralizing Antibody
Erika Takemasa, Shuang Liu, and Hitoshi Hasegawa
10. Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen
Hiroyuki Takeda
11. Generation of Specific Aptamers
Shuang Liu, Yasuyuki Suzuki, and Makoto Inui
12. Production of Lentiviral Particles
Shuang Liu
13. RNA Interference Ex Vivo
Shuang Liu
14. Lentiviral-mediated Systemic RNA Interference in vivo
Shuang Liu
15. Mesenchymal Stem Cell Engineering
Shuang Liu
Part III: Evaluation of Immunological Status
16. Screening of Ca2+ Influx in Lymphocytes Erika Takemasa and Shuang Liu
17. Single-cell Ca2+ Imaging
Shuang Liu
18. Electrophysiological Methods to Measure Ca2+ Current
Shuang Liu and Miyuki Kuno
19. The Functional Assessment of T cells
Saho Maruyama
20. Release of Antibodies and Cytokines from B cells
Shuang Liu
21. Evaluation of Autoreactive Responses
Shaung Liu
22. Bone-resorption Activity in Mature Osteoclasts Takeshi Kyoi
23. Animal Models of Vasculitis
Mogi M and Shuang Liu
Part IV: Clinical Approach
24. Design an Intervention Study
Hitoshi Hasegawa
25. Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis
Hitoshi Hasegawa
