Part I-Structural RNA Mapping
1. Full-Length Characterization Of Transcribed Genomic Regions
Marc René Reboll, M. Lucrecia Alvarez, and Mahtab Nourbakhsh
2. Rapid Mapping Of RNA 3´and 5´Ends
Victoria Zismann and Mahtab Nourbakhsh
3. Single Nucleotide Mapping Of RNA 5´and 3´Ends
Mahtab Nourbakhsh
4. RNA Secondary Structure Analysis
Mahtab Nourbakhsh
5. Tertiary Structure Mapping Of Pri-Mirna17 92
Steven G. Chaulk and Richard P. Fahlman
6. In Situ Hybridization Detection Of Micrornas Using LNA Oligonucleotides
Olga Beltcheva and Stefania Cotta Done
7. Quantification Of Micrornas By A Simple And Specific Qpcr Method Peter Busk and Susanna Cirera
8. RNA Isolation For Small RNA Next Generation Sequencing From Acellular Biofluids
Kasandra L. Burgos and Kendall Van Keuren-Jensen
9. Sequencing Small RNA: Introduction and Data Analysis Fundamentals
Jai Prakash Mehta
10. Measuring Expression Levels Of Small Regulatory RNA Molecules From Formalin-Fixed, Paraffin-Embedded Samples and Body Fluids
Adrienn Gyongyosi, Otto Docs, Zsolt Czimmerer, Laszlo Orosz, Attila Horvath, Olga Török, Gabor Mehes, Laszlo Nagy, and Balint L Balint
11. Microrna Profiling In Plasma Or Serum Using Quantitative RT-Qpcr
Marina C. Costa, Ana Lúcia Leitão, and Francisco J. Enguita
12. Microrna Profiling Of Exosomes Isolated From Biofluids And Conditioned Media
Sweta Rani
13. Isolation Of Urinary Exosomes For RNA Biomarker Discovery Using A Simple, Fast, And Highly Scalable Method
M. Lucrecia Alvarez
Part II- Functional RNA Mapping
14. Identification Of Actively Translated Mrnas
Marc René Reboll and Mahtab Nourbakhsh
15. Mapping Of Internal Ribosome Entry Sites (IRES)
Sarah Mehrtens and Marc René Reboll
16. Mapping Of Protein Binding RNA Elements
Marc René Reboll
17. Purification Of RNA Binding Proteins
Birgit Ritter and Marc René Reboll
18. De Novo Approach To Classify Protein-Coding And Non-Coding Transcripts By Utilizing Sequence Intrinsic Composition
Haitao Luo, Dechao Bu, Liang Sun, and Yi Zhao
19. Computational Methods To Predict Long Non-Coding RNA Functions Based On Co-Expression Network
Yi Zhao, Haitao Luo, Xiaowei Chen, Yi Xiao, and Runsheng Chen
20. Microrna Biogenesis - Dicing Assay
Carlos A. Melo and Sonia A. Melo
21. Faster Experimental Validation Of Microrna Targets Using Cold Fusion Cloning And A Dual Firefly-Renilla Luciferase Reporter Assay
M. Lucrecia Alvarez
22. Experimental Validation Of Predicted Mammalian Micrornas Of Mirtron Origin
Anita Schamberger and Tamás I. Orbán
23. A Guide For Microrna Target Prediction And Analysis Using Web-Based Applications
Ana Lúcia Leitão, Marina C. Costa, and Francisco J. Enguita
24. Tapping Microrna Regulation Networks Through Integrated Analysis Of Microrna-Mrna High-Throughput Profiles
Anthony D. Saleh and Hui Cheng
25. Mirwalk Database For Microrna-Target Interactions
Harsh Dweep, Norbert Gretz And Carsten Sticht
26. A Schematic Workflow For Collecting The Interaction Information Between Copy Number Variants And Micrornas Using Existing Resources
Harsh Dweep, Norbert Gretz, and Kyriakos Felekkis
27. SYBR Green and Taqman PCR Arrays: Expression Profile Of Genes Relevant To A Pathway Or Disease State
M. Lucrecia Alvarez And Stefania Cotta Done
28. Comprehensive Meta-Analysis Of Microrna Expression Using A Robust Rank Aggregation Approach
Urmo Võsa, Raivo Kolde, Jaak Vilo, Andres Met
