Part I: Genetic and Phenotypic Analyses
1. Measuring Plant Root Traits Under Controlled and Field Conditions: Step-By-Step Procedures
Benjamin M. Delory, Emanuela W.A. Weidlich, Richard van Duijnen, Loïc Pagès, and Vicky M. Temperton
2. Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean
Larry M. York
3. Developmental Analysis of Arabidopsis Root Meristem
Riccardo Di Mambro and Sabrina Sabatini
4. Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana
Selene Napsucialy-Mendivil and Joseph G. Dubrovsky
5. Adapting the Lateral Root Inducible System to Medicago truncatula
Violaine Herrbach, Fabienne Maillet, and Sandra Bensmihen
6. Characterization of Root Epidermal Cell Patterning and differentiation in Arabidopsis
Jorge E. Salazar-Henao, Girish Mokkapati, Eunice Han Xian Khor, Yi-Chia Chou, Wann-Neng Jane, and Wolfgang Schmidt
7. In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls
Hoang Khai Trinh, Inge Verstraeten, and Danny Geelen
8. Root Gravitropism: Quantification, Challenges, and Solutions
Lukas Muller, Malcolm J. Bennett, Andy French, Darren M. Wells, and Ranjan Swarup
Part II: Microscopy and Cell Biology
9. Calcium Ion Dynamics in Roots: Imaging and Analysis
Ellie Himschoot, Melanie Krebs, Alex Costa, Tom Beeckman, and Steffen Vanneste
10. Optimized Whole-Mount In Situ Immunolocalization for Arabidopsis thaliana Root Meristems and Lateral Root Primordia
Michael Karampelias, Ricardo Tejos, Jiří Friml, and Steffen Vanneste
11. --Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development
Paolo Baesso, Ricardo S. Randall, and Giovanni Sena
12. Histological Profiling Over Time to Optimize Root Cell Type-Specific Reporter Lines for Cell Sorting
Beatriz Lagunas, Liam Walker, Rana Muhammad Fraz Hussain, Ian Hands-Portman, Kate Woolley-Allen, and Miriam L. Gifford
13. Long-Term In Vivo Imaging of Luciferase-Based Reporter Gene Expression in Arabidopsis Roots
Wei Xuan, Davy Opdenacker, Steffen Vanneste, and Tom Beeckman
14. Cortical Cell Length Analysis During Gravitropic Root Growth
Maria Schöller, Jürgen Kleine-Vehn, and Elena Feraru
15. Growth Rate Normalization Method to Assess Gravitropic Root Growth
Maria Sch