1. Preparation of Solutions and Reagents
Teresa L. Singleton
2. Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing
Atanas Kaykov
3. Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
Valerie Migeot and Damien Hermand
4. Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs Fortunato Ferrara, Andrew R. M. Bradbury, and Sara D'Angelo
5. Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
Ge Li and Richard Y. Zhao
6. Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
Robert Roth, Hiten D. Madhani, and Jennifer F. Garcia
7. Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis
Pranjal Biswas, Uddalak Majumdar, and Sanjay Ghosh
8. The No-nonsens SDS-PAGE
Lars Backman and Karina Persson
9. Crystallization of Recombinant α-actinin and Related Proteins
Karina Persson and Lars Backman
10. Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy
Haruhiko Asakawa, Yasushi Hiraoka, and Tokuko Haraguchi
11. Antibody Pull-down Experiments in Fission Yeast
Qianhua Dong and Fei Li
12. Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
Akihisa Matsuyama, Atsuko Shirai, and Minoru Yoshida
13. Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
Zhen Zhu, Olivier Frey, and Andreas Hierlemann
14. In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe
Jinpu Yang and Fei Li
15. High-frequency Lithium Acetate Transformation of Schizosaccharomyces pombe
Sudhir Kumar Rai, Angela Atwood-Moore, and Henry L Levin
16. Tetrad Dissection in Fission Yeast
Wilber Escorcia and Susan L. Forsburg
17. Random Spore Analysis in Fission Yeast
Wilber Escorcia and Susan L. Forsburg
18. Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
Sudhir Kumar Rai, Angela Atwood-Moore, and Henry L. Levin
19. Schizosaccharomyces pombe Biotechnological Applications in Winemaking
Ángel Benito, Fernando Calderón, and Santiago Benito
20. Schizosaccharomyces pombe Isolation Protocol
Ángel Benito, Fernando Calderón, and Santiago Benito