Part I Updated Blood Processing and Handling Strategies

1. Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation

Viviana Greco, Cristian Piras, Luisa Pieroni, and Andrea Urbani

2. A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma

Rosemary L Sparrow, David W Greening, and Richard J Simpson

3. A Proteomic Protocol for the Preparation of Platelet Concentrates

David W Greening, Richard J Simpson, and Rosemary L Sparrow

Part II Updated Fractionation Strategies for In-depth Blood Proteome Analysis

4. Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture

Elin Birgersson, Jochen M. Schwenk, and Burcu Ayoglu

5. Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma

John P O'Grady, Kevin W Meyer, and Derrick N Poe

6. Characterization of the Low-molecular Weight Human Plasma Peptidome

David W. Greening and Richard J. Simpson

7. In-depth, Reproducible Analysis of Human Plasma using IgY 14 and SuperMix Immunodepletion

Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher

8. Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry

Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, and Ji Eun Lee

9. Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis without Depletion of Abundant Proteins

10. Identification of Core-fucosylated Glycoproteome in Human Plasma

Qichen Cao, Qing Zhao, Xiaohong Qian, and Wantao Ying

Part III Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids

11. Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis

12. Targeted Approach For Proteomic Analysis of a Hidden Membrane Protein

Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, and Henrique Girao

13. Red Blood Cells in Clinical Proteomics

Ana Sofia Carvalho, Manuel S Rodriguez, and Rune Matthiesen

14. High-throughput Quantitative Lipidomics Analysis of Non-esterified Fatty Acids in Plasma by LC-MS

Nicolas Christinat, Delphine Morin-Rivron, and Mojgan Masoodi

15. Simultaneous Enrichment of Plasma Extracellular Vesicles and Glycoproteome for Studying Disease Biomarkers

Sunil S. Adav and Siu Kwan Sze

Susanne Sales, Oskar Knittelfelder, and Andrej Shevchenko

17. Proteomics Analysis of Circulating Serum Exosomes

Antonius Koller, Purvi Patel, Jenny Kim Kim, and Emily I. Chen

Part IV Antibody-based Discovery and Targeted Proteomics

18. High-density Serum/Plasma Reverse Phase Protein Arrays

Cecilia Hellström, Tea Dodig-Crnkovic, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, and Ronald Sjöberg

19. Antibody Colocalization Microarray for Cross-reactivity free Multiplexed Protein Analysis

Véronique Laforte, Pik-Shan Lo, Huiyan Li, and David Juncker

20. Surface Profiling of Extracellular Vesicles From Plasma or Ascites Fluid using Dotscan Antibody Microarrays