About the Book
1. Single-Cell Western Blotting
Elly Sinkala and Amy E. Herr
2. A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. Coli Bacteria
Simone Stratz and Petra S. Dittrich
3. Enzyme-Linked ImmunoSpot (ELISpot) for Single Cell Analysis
Sylvia Janetzki and Rachel Rabin
4. Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells
Adeeti V. Ullal and Ralph Weissleder
5. Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia Coli Cells with Single-Molecule Sensitivity
Yuichi Taniguchi
6. Microfluidic Flow Cytometry for Single Cell Protein Analysis
Meiye Wu and Anup K. Singh
7. Microfluidic Image Cytometry for Single Cell Phenotyping of Human Pluripotent Stem Cells
Yasumasa Mashimo and Ken-Ichiro Kamei
8. Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry
Nalin Leelatian, Kirsten E. Diggins, and Jonathan M. Irish
9. Multiplex Peptide-MHC Tetramer Staining with Mass Cytometry
Leong Mei Ling and Evan W. Newell
10. Imaging and Mapping of Tissue Constituents at the Single Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry
Catherine M. Rawlins, Joseph P. Salisbury, Daniel R. Feldman, Sinan Isim, Nathalie Y.R. Agar, Ed Luther, and Jeffery N. Agar
11. SPLIFF: A Single Cell Method to Map Protein-Protein Interactions in Time and Space
Alexander Dünkler, Reinhild Rösler, Hans A. Kestler, Daniel Moreno, and Nils Johnsson
12. Microfluidic Proximity Ligation Assay for Profiling Signaling
Networks with Single-Cell Resolution
Matthias Blazek, Roland Zengerle, and Matthias Meier13. Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
Stephen Anthony, Amanda Carroll-Portillo, and Jerilyn Timlin
14. Microfluidics Enabled Enzyme Activity Measurement in Single Cells
Cinzia Tesauro, Rikke Frøhlich, Magnus Stougaard, Yi-Ping Ho, and Birgitta R. Knudsen
15. Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
Livia Shehaj, Lorena Lazo de la Vega, and Michelle L. Kovarik
16. Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells
Zhenning Cao and Chang Lu
About the Author: Anup Singh Mailstop 9291 Sandia National Laboratories 7011 East Ave Livermore, CA aksingh@sandia.gov