Part I
1. Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions
Joost van Mameren, Gijs J. L. Wuite, and Iddo Heller
2. RNA Unzipping and Force Measurements with a Dual Optical Trap
Laurent Geffroy, Pierre Mangeol, Thierry Bizebard, and Ulrich Bockelmann
3. Protein Tethering for Folding Studies
Fatemeh Moayed, Roeland J. van Wijk, David P. Minde, and Sander J. Tans
4. Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein
Lu Rao, Maren Hülsemann, and Arne Gennerich
5. A Brief Introduction to Single-Molecule Fluorescence Methods
Siet M. J. L. van den Wildenberg, Bram Prevo, and Erwin J. G. Peterman
PART II
6. Fluorescent Labeling of Proteins
Mauro Modesti
7. Single-Molecule Imaging of Escherichia coli Trans-Membrane Proteins
Aravindan Varadarajan, Felix Oswald, and Yves J. M. Bollen
8. Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans
Jaap van Krugten and Erwin J. G. Peterman
9. Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy
Roderick P. Tas, Trusanne G. A. A. Bos, and Lukas C. Kapitein
10. Fluorescence Microscopy of Nanochannel-Confined DNA
Fredrik Westerlund, Fredrik Persson, Joachim Fritsche, Jason P. Beech, and Jonas O. Tegenfeldt
11. Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin--Microtubule Complexes
Matthieu P. M. H. Benoit and Hernando Sosa
12. Single Molecule FRET Analysis of DNA Binding Proteins
Kathy R. Chaurasiya and Remus T. Dame
PART III 13. Atomic Force Microscopy: An Introduction
Melissa C. Piontek and Wouter H. Roos
14. Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy
Malgorzata Grosbart, Dejan Ristic, Humberto Sánchez, and Claire Wyman
15. Atomic Force Microscopy of Protein Shells: Virus Capsids and Beyond
Alvaro Ortega-Esteban, N. Martín-González, F. Moreno-Madrid, Aida Llauró, Mercedes Hernando-Pérez, and Pedro J. de Pablo
16. Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single Molecule Manipulation and Visualization
Yeonee Seol and Keir C. Neuman
17. Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Transcription Factor Interactions
Daniel T. Tovari, Yan Yan, Laura Finzi, and David Dunlap
18. Single-Molecule Measurements Using Acoustic Force Spectroscopy (AFS)
Douwe Kamsma, and Gijs J.L. Wuite
19. Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy
Daren Yang, and Wesley P. Wong
