Skeletal Muscle Development

Part I: Skeletal Muscle Gene Expression

1. Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells

Kohei Miyata, Masashi Naito, Tomoko Miyata, Sho Mokuda, and Hiroshi Asahara

2. Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells

Karl Kamhei So, Xianlu Peng, Hao Sun, and Huating Wang

3. Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle

Tamar E. Sztal, Peter D. Currie, and Robert J. Bryson-Richardson

4. Chick Somite Electroporation for Gene Function Studies

Muhammad Abu-Elmagd

Part II: Assays for Proliferating Skeletal Muscle Cells

5. Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS

6. Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells

C. Hai Ly and James G. Ryall

7. Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures

Victor M. Aguilar and Benjamin D. Cosgrove

8. Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle

9. Human Satellite Cell Isolation and Xenotransplantation

Steven M. Garcia, Stanley Tamaki, Xiaoti Xu, and Jason H. Pomerantz

Part III: Assessment of Myogenic Differentiation

10. Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro

Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, and Yutaka Hata

11. Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells

Toshifumi Asano, Toru Ishizuka, and Hiromu Yawo

12. Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes

Gio C. Suh, Archana Bettadapur, Jeffrey W. Santoso, and Megan L. McCain

Part IV: Assessing Skeletal Muscle Development In Vivo

13. Quantification of Embryonic Myofiber Development by Immunofluorescence

Harika Nagandla and M. David Stewart

14. How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation

Part V: Muscle Repair Assays

15. Membrane Repair Assay for Human Skeletal Muscle Cells

Romain Carmeille, Coralie Croissant, Flora Bouvet, and Anthony Bouter

16. Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle