Section I. Semen Analysis Methods
1. Methods For Sperm Concentration Determination
Lars Björndahl
2. Methods of Sperm Vitality Assessment
Sergey I. Moskovtsev and Clifford L. Librach
3. The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity
Sivakumar Ramu and Rajasingam S. Jeyendran
4. Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization
Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow
5. Sperm Morphology Assessment Using Strict (Tygerberg) Criteria
Roelof Menkveld
6. Methods For Direct And Indirect Antisperm Antibody Testing
Hiroaki Shibahara and Junko Koriyama
7. Manual Methods For Sperm Motility Assessment
David Mortimer and Sharon T. Mortimer
8. Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation
David Mortimer and Sharon T. Mortimer
Section II. Tests of Sperm Function
9. The Hemizona Assay For Assessment Of Sperm Function
Sergio Oehninger, Mahmood Morshedi, and Daniel Franken
10. The Sperm Penetration Assay for the Assessment of Fertilization Capacity
Kathleen Hwang and Dolores J. Lamb
11. Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay
Christopher J. De Jonge and Christopher L. R. Barratt
Section III. Sperm DNA Damage Testing
12. Sperm DNA Fragmentation Analysis Using the TUNEL Assay
Rakesh Sharma, Jayson Masaki, and Ashok Agarwal
13. Sperm DNA Damage Measured by Comet Assay
Luke Simon and Douglas T. Carrell
14. Sperm Chromatin Structure Assay (SCSA(R))
Donald P. Evenson
Section IV. Advanced Clinical Testing
15. Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH)
Benjamin R. Emery
16. Flow Cytometric Methods For Sperm Assessment
Vanesa Robles and Felipe Martínez-Pastor
17. Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions
Peter H. Vogt and Ulrike Bender
Section V. Sperm Preparation and Selection Techniques
18. Sperm Cryopreservation Methods
Tiffany Justice and Greg Christensen
19. Density Gradient Separation Of Sperm For Artificial Insemination
David Mortimer and Sharon T. Mortimer
20. Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis
Charles H. Muller and Erin R. Pagel
21. Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor
Shilo L. Archer and William E. Roudebush
22. Intracytoplasmic Morphology Selected Sperm Injection
Luke Simon, Aaron Wilcox, and Douglas T. Carrell
23. Sperm Selection For ICSI Using Annexin V
Sonja Grunewald and Uwe Paasch
24. Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay
Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh
25. Sperm Selection Based On Electrostatic Charge
Luke Simon, Shao-qin Ge, and Douglas T. Carrell
26. Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting
Duane L. Garner, K. Michael Evans, and George E. Seidel, Jr.
Section VI. Staging and Histology Techniques