1. Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay
Jonathan K. Fleming and Jonathan M. Wojciak
2. An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity
Melissa R. Pitman, Lorena T. Davies, and Stuart M. Pitson
3. Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells
Alena P. Chumanevich, Piper A. Wedman, and Carole A. Oskeritzian
4. Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry
Maria P. Kraemer, Suchismita Halder, Susan S. Smyth, and Andrew J. Morris
5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines
Gary M. Reynolds, Barbara Visentin, and Roger Sabbadini
6. A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule Jun-ichi Morishige, Ryouhei Yamashita, Tamotsu Tanaka, and Kiyoshi Satouchi
7. A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes
Naoki Kobayashi and Tsuyoshi Nishi
8. Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry
Jianhong Zhang, Annie Bang, and Stephen J. Lye
9. S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses
Camille Duran, Roland Kaunas, and Kayla Bayless
10. In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate
Melina G. Castro, Ludmila E. Campos, Yamila I. Rodriguez, and Sergio E. Alvarez
11. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor
Raymond C.B. Wong, Martin F. Pera, and Alice Pébay
12. Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors
Phillip Callihan, Mohammed Alqinyah, and Shelley B. Hooks
13. Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation
Guanghu Wang, Stefka D. Spassieva, and Erhard Bieberich
14. 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering
Shrestha Priyadarsini, Sarah E. Nicholas, and Dimitrios Karamichos