1. Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate
Brizuela Leyre and Cuvillier Olivier
2. Isoform-Selective Assays for Sphingosine Kinase Activity
Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson
3. Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry
Constantin Bode and Markus H. Gräler
4. A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule
Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi
5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples
Barbara Visentin, Gary Reynolds, and Roger Sabbadini
6. Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation
Hideru Obinata and Timothy Hla
7. High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes
William J. Valentine and Gabor Tigyi
8. Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases
Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack
9. Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm
Silvia A. Belmonte and Laila Suhaiman
10. Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis
Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii
11. Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System
Veronique E. Miron, Bryce A. Durafourt, Jack P. Antel, and Timothy E. Kennedy
12. Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells
Elisabetta Meacci, Francesca Bini, and Chiara Battistini
13. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor
Raymond C.B. Wong, Martin F. Pera, and Alice Pébay
14. Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation
Erhard Bieberich
15. Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells
He Xiaoli, Sathish Jean, and Alirio J. Melendez
16. Sphingosine-1-Phosphate Signaling in Neural Progenitors
Phillip Callihan and Shelley B. Hooks
17. Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis
Shih-Chi Su and Kayla J. Bayless
