About the Book
1. The Heterogeneity of Stem Cells: A Brief Overview
Györgyi Műzes and Ferenc Sipos
2. Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells
Sharat Warrier, Mina Popovic, Margot Van der Jeught, and Björn Heindryckx
3. An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells
Guoliang Meng, Anna Poon, Shiying Liu, and Derrick E. Rancourt
4. Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter
Leonid V. Bystrykh and Mirjam E. Belderbos
5. Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells
Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak
6. Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells
Ana M.V. Guedes, Domingos Henrique, and Elsa Abranches
7. Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)
Josh Cutts, Nicholas Brookhouser, and David. A Brafman
8. Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice
Srabani Kar, Shinjini Mitra, and Ena Ray Banerjee
9. Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing
Amar M. Singh, Dustin W. Perry, Valeriya V. Adjan Steffey, Kenneth Miller, and Daniel W. Allison
10. Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity
Jun Yuan, Geetika Sahni, and Yi-Chin Toh
11. Visualizing the Functional Heterogeneity of Muscle Stem Cells
Yasuo Kitajima, Shizuka Ogawa, and Yusuke Ono
12. Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies
Chiara Franzin, Martina Piccoli, Luca Urbani, Carlo Biz, Piergiorgio Gamba, Paolo De Coppi, and Michela Pozzobon
13. Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells
Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak
14. Growth Factor-Free Pre-Vascularization of Cell Sheets for Tissue Engineering
Marina Costa, Rogério P. Pirraco, Mariana T. Cerqueira, Rui L. Reis, and Alexandra P. Marques
15. In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry
Marc Cloutier, Christine Jobin, Carl Simard, and Sonia Néron
16. Aerosol-Based