About the Book

A testimony on the use of Stem Cells to discover and develop new therapeutic approaches to control variety of ocular diseases.
• Delivers in-depth understanding of the present situation of research in different subfields of Ophthalmology.
• Reviews basic stem cells biology and immunity.
• The final chapter covers description of the facilities and governmental requirements needed to establish an ocular stem cell program.
• Discusses unique advances and state-of-the-art use of stem cells in ocular tissue.
• Written and contributed by excellent clinical and surgical ophthalmologists from around the globe.
• It will be a “go to” resource in the planning of future research endeavours in stem cells.

About the Author

Daniel H. Scorsetti MD, PhD Victor L. Perez MD Jose Alvaro Pereira Gomes MD, PhD

Table of Contents:
CONTENTS Chapter 1: Stem Cells...................................................................................... 1 Enrique Salero, PhD Daniel Pelaez, PhD Chapter 2: Ocular Surface Epithelia Side Population Stem Cells: Isolation, Molecular Signatures and Approaches to Activation and Self Renewal for Tissue Regeneration............... 15 Alex Barash, MD Ozlem Barut Selver, MD So-Hyang Chung, MD Zhen Wang, MD Ashton Shiraz, MD Peter S. Reinach, PhD J. Mario Wolosin, PhD Chapter 3: Cultivation In Vitro and Ex Vivo................................................... 37 Virender S. Sangwan, MD Praful Kumar, MD Ikeda Lal, MD, FICO Namrata Sharma, MD Chapter 4: Avoiding Rejection: Immunological Aspects for Successful Stem Cell Therapy...................................................... 51 Fernando Cruz-Guilloty, PhD Victor L. Perez, MD Chapter 5: Ocular Surface Stem Cells: Science and Surgery........................ 61 Harminder S. Dua, MD, PhD Aaron Yeung, MD, PhD Dalia G. Said, MD, FRCS Chapter 6: Corneal Stromal Keratocytes and Endothelial Cells................... 81 Gary Hin-Fai Yam, MD Matthew Jason Lovatt, MD Gary Swee-Lim Peh, MD Donald Tan, MD Jodhbir Singh Mehta, MDxiv STEM CELLS IN OPHTHALMOLOGY Chapter 7: Restricted Pluripotent Cells and Their Integration into the Trabecular Meshwork.............................................................. 93 Cameron Pole, BS Richard K. Lee, MD, PhD Sanjoy K. Bhattacharya, PhD Chapter 8: Crystalline Lens Stem Cells, Reparation and Regeneration....... 101 Roberto A. Cohen, MD Juan O. Croxatto, MD Daniel H. Scorsetti, MD, PhD Chapter 9: Stem Cells in the Orbit and Ocular Adnexal Tissue................... 109 Sara T. Wester, MD Chapter 10: Cell Therapy for Total Limbal Stem Cell Deficiency................... 125 Jose Reinaldo da Silva Ricardo, MD, PhD Priscila Cardoso Cristovam, PhD Babyla Geraldes Monteiro, BHS Rubens Belfort Jr., MD, PhD Irina Kerkis, PhD Jose Alvaro Pereira Gomes, MD, PhD Chapter 11: Cell Therapy for Corneal Endothelial Dysfunction.................... 135 Noriko Koizumi, MD Naoki Okumura, MD Shigeru Kinoshita, MD Chapter 12: Cell Therapy for Retinal, Optic Nerve and Visual Tract Diseases.................................................................... 149 Rodrigo A. Brant Fernandes, MD Bruno Diniz, MD Ramiro Ribeiro, MD Fernando M. Penha, MD, PhD Yuntao Hu, MD, PhD Mauricio Maia, MD, PhD Mark Humayun, MD, PhD Caio V. Regatieri, MD, PhDxv Chapter 13: Role of Stem Cells in Lacrimal and Meibomian Gland Development and Regeneration.................................................. 165 Driss Zoukhri, PhD Helen P. Makarenkova, PhD Chapter 14: Towards the Use of Non-Ocular Cell Lineages in Ocular Surface Disease................................................................. 179 Johannes Menzel-Severing, MD, MSc Naresh Polisetti, PhD Ursula Schlötzer-Schrehardt, PhD Friedrich E. Kruse, MD Chapter 15: Induced Pluripotent Stem Cells and Their Potential to Treat Eye Diseases................................................................... 187 Fernando Cruz-Guilloty, PhD Victor L. Perez, MD Chapter 16: Gene Therapy................................................................................ 195 Medi Eslani, MD Asadolah Movahedan, MD Ali R. Djalilian, MD Chapter 17: Protocols, Facilities, Equipment, Materials and Regulations for Establishing a Human Stem Cell Program....... 217 Jose J. Echegaray, MD, PhD Fernando Cruz-Guilloty, PhD Priscila Cardoso Cristovam, PhD José Alvaro P. Gomes, MD, PhD Victor L. Perez, MD Index....................................................................................................................... 227