Part I. Cloning, Expression and Protein Production

1. DisMeta - a Meta Server for Construct Design and Optimization

Yuanpeng Janet Huang, Thomas B. Acton, and Gaetano T. Montelione

2. Stable Expression Clones and Auto-Induction for Protein Production in E. Coli

F. William Studier

3. High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli

Natalie J. Saez and Renaud Vincentelli

4. Medium Throughput Production of Recombinant Human Proteins: Ligation Independent Cloning

Claire Strain-Damerell, Nicola A. Burgess-Brown, Pravin Mahajan, and Opher Gileadi

5. Medium Throughput Production of Recombinant Human Proteins: Protein Production in E. coli

Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi, and Susanne Gräslund

6. Medium Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells

Pravin Mahajan, Nicola A. Burgess-Brown, Claire Strain-Damerell, and Opher Gileadi

7. OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems

Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, and Imre Berger

8. Multiprotein Complex Production in Insect Cells by Using Polyproteins

Yan Nie, Itxaso Bellon-Echeverria, Simon Trowitzsch, Christoph Bieniossek, and Imre Berger

9. Expression Screening in Mammalian Suspension Cells

Susan D. Chapple and Michael R. Dyson

10. Cell-free Expression of Protein Complexes for Structural Biology

Takaho Terada, Takeshi Murata, Mikako Shirouzu, and Shigeyuki Yokoyama

11. Cell-Free Protein Synthesis for Functional and Structural Studies

Shin-ichi Makino, Emily T. Beebe, John L. Markley, and Brian G. Fox

12. Insoluble Protein Purification with Sarkosyl: Facts and Precautions

Ben Chisnall, Courtney Johnson, Yavuz Kulaberoglu, and Yu Wai Chen

Part II. Experimental Structure Determination and Characterisation

13. Estimation of Crystallization Likelihood Through a Fluorimetric Thermal Stability Assay

Vincent Mariaule, Florine Dupeus, and José A. Márquez

14. CrystalDirect(TM) A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films

Jose A. Marquez and Florent Cipriani

15. Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropy

Michael R. Sawaya

16. Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination

Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, and Michael A. Kennedy

17. A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labelled Mutants for NMR Studies of High Molecular Weight Proteins

Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet, and Jerome Boisbouvier

18. High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach

Kevin N. Dyer, Michal Hammel, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer, and Greg L. Hura

19. Measuring Spatial Restraints on Native Protein Complexes using Isotope-Tagged Chemical Cross-Linking and Mass Spectrometry

Franz Herzog

Part III. Computational Methods and Structural Data Analyses

20. Modeling of Proteins and Their Assemblies with the Integrative Modeling Platform

Benjamin Webb, Ker