About the Book
Part I: Amino Acid-Based Synthetic Antibodies
1. Antibody Mimetics, Peptides, and Peptidomimetics
Xiaoying Zhang and Thirumalai Diraviyam
2. Construction of a scFv Library with Synthetic, Non-Combinatorial CDR Diversity
Xuelian Bai and Hyunbo Shim
3. Enzymatic Assembly for scFv Library Construction
Mieko Kato and Yoshiro Hanyu
4. Directed Evolution of Protein Thermal Stability Using Yeast Surface Display
Michael W. Traxlmayr and Eric V. Shusta
5. Whole Cell Panning with Phage Display
Yvonne Stark, Sophie Venet, and Annika Schmid
6. Generating Conformation and Complex-Specific Synthetic Antibodies
Marcin Paduch and Anthony A. Kossiakoff
7. High-Throughput IgG Conversion of Phage Displayed Fab Antibody Fragments by AmplYFast
Andrea Sterner and Carolin Zehetmeier
8. Utilization of Selenocysteine for Site-Specific Antibody Conjugation
Xiuling Li and Christoph Rader
9. Solubility Characterization and Imaging of Intrabodies Using GFP-Fusions
Emilie Renaud, Pierre Martineau, and Laurence Guglielmi
10. Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis
Helena Persson, Charlotta Preger, Edyta Marcon, Johan Lengqvist, and Susanne Gräslund
11. Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules
Neeraj Kohli and Melissa L. Geddie
12. Glycosylation Profiling of α/β T Cell Receptor Constant Domains Expressed in Mammalian Cells
Kai Zhang, Stephen J. Demarest, Xiufeng Wu, and Jonathan R. Fitchett
13. A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells
Hongkai Zhang and Richard A. Lerner
14. A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins
Wouter P.R. Verdurmen, Marigona Mazlami, and Andreas Plückthun
15. Data-Driven Antibody Engineering Using Genedata Biologics(TM)
Maria Wendt and Guido Cappuccilli
Part II: Nucleotide-Based Synthetic Antibodies: Aptamers
16. Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers
Nam Nguyen Quang, Anna Miodek, Agnes Cibiel, and Frédéric Ducongé
17. Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules
