Part I: Background Information
1. 200 Million Thymocytes and I: A Beginner's Survival Guide to T cell Development
Melanie S. Vacchio, Thomas Ciucci and Rémy Bosselut
2. Development of gd T Cells, the Special Forces Soldiers of the Immune System
David L. Wiest
Part II: Core Approaches and Strategies
3. Genetic Tools to Study T Cell Development
Thomas Ciucci, Melanie S. Vacchio and Rémy Bosselut
4. Assessment of T cell Development by Flow Cytometry
Jan Y.M. Lee and Paul E. Love
5. Flow Cytometry Analysis of Thymic Cells and their Subpopulations
Izumi Ohigashi and Yousuke Takahama Epithelial
6. Identifying the Spatial Relationships of Thymic Stromal and Thymocyte Subsets by Immunofluorescence Analysis
Virginia Bain and Ellen R. Richie
7. Purification of Thymocyte and T Cell Subsets
Andrea C. Carpenter, Jong Kyong Kim, and Rémy Bosselut
8. Retroviral Transduction of T Cells and T Cell Precursors
Amie Simmons and José Alberola-Ila
9. Bone Marrow and Fetal Liver Radiation Chimeras
Francis A Flomerfelt and Ronald E Gress
10. In vitro Analyses of T Cell Effector Differentiation
Elizabeth A. Wohlfert,
Andrea C. Carpenter, Yasmine Belkaid, and Rémy Bosselut
Part III: Specific Techniques
11. Studying T Cell Development in Thymic Slices
Jenny O. Ross, Heather J. Melichar, Joanna Halkias, and Ellen A. Robey
12. FTOC-based Analysis of Negative Selection
Cody, A. Cunningham, Emma Teixeiro, and Mark A. Daniels
13. Reconstituted Thymus Organ Culture
Zimu Deng, Haifeng Liu, Jinxiu Rui, and Xiaolong Liu
14. Induction of T-cell Development in vitro by Delta-like (Dll)-Expressing Stromal Cells
Mahmood Mohtashami, Payam Zarin and Juan Carlos Zúñiga-Pflücker
15. In vitro Analysis of Thymocyte Signaling
Cody A. Cunningham, Emma Teixeiro, Mark A. Daniels
16. Molecular Analysis of Mouse T Cell Receptor a and b Gene Rearrangements
Levi J. Rupp, Liang Chen, Michael S. Krangel, and Craig H. Bassing
17. Intrathymic Injection
Sugata Manna and Avinash Bhandoola
18. Analysis of Cell Proliferation and Homeostasis using EdU Labeling
Francis A Flomerfelt and Ronald E Gress
19.
Characterization and Isolation of Human T Cell ProgenitorsKarin Davids, Inge Van de Walle and Tom Taghon
20. Approaches to Study Human T Cell Development
Anne-Catherine Dolens, Inge Van de Walle and Tom Taghon
21. Humanized Mice to Study Human T Cell Development
Sarah Bonte, Sylvia Snauwaert, Stijn Vanhee, Anne-Catherine Dolens, Tom Taghon, Bart Vandekerckhove, and Tessa Kerre
22. Using the Zebrafish Model to Study T cell Development
Yong Zhang and David L. Wiest
