About the Book
1. Regulation of TGF-b Receptors
Erine Budi, Jian Xu and Rik Derynck
2. Determining TGF-β Receptor Levels in the Cell Membrane
Long Zhang, Fangfang Zhou, Maarten van Dinther, and Peter ten Dijke
3. Post-translational Modifications of TGF-b Receptors
Xiaohua Yan and Ye-Guang Chen
4. Production, Isolation，and Structural Analysis of Ligands and Receptors of the TGF-b Superfamily
Tao Huang and Andrew P. Hinck
5. Phosphorylation of Smads by Intracellular Kinases
Fang Liu and Isao Matsuura
6. Analysis of Smad Phosphatase Activity in vitro
Tao Shen, Lan Qin and Xia Lin
7. Three-dimensional Mammary Epithelial Cell Morphogenesis Model for Analysis of TGF-b Signaling
Juliet Rashidian and Kunxin Luo
8. TGF-β Signaling in Stem Cell Regulation
Wenlin Li, Wanguo Wei, Sheng Ding
9. Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor β
Ulrich Valcourt, Jonathon Carthy, Yukari Okita, Lindsay Alcaraz, Mitsuyasu Kato, Sylvie Thuault, Laurent Bartholin and Aristidis Moustakas
10. In vitro Th differentiation protocol
Takashi Sekiya and Akihiko Yoshimura
11. Interrogating TGF-b Function and Regulation in Endothelial Cells
J.A. Maring, L.A. van Meeteren, M.J. Goumans and P. ten Dijke
12. Isolation and Manipulation of Adipogenic Cells to Assess TGF-b Superfamily Functions
Maria Namwanje, Juan .C. Bournat and Chester .W. Brown
13. Imaging TGFb Signaling in Mouse Models of Cancer Metastasis
Yibin Kang
14. Generation and Characterization of Smad7 Conditional Knockout Mice
Yi Pan and Yan Chen
15. Monitoring Smad Activity in vivo Using the Xenopus Model System
Marco Montagner, Graziano Martello and Stefano Piccolo
16. Animal Cap Assay for TGF-b Signaling
Chenbei Chang
17. Detection of Smad Signaling in Zebrafish Embryos
Xingfeng Liu, Qiang Wang and Anming Meng
18. Role of TGF-β signaling in Coupling Bone Remodeling
Janet L. Crane, Lingling Xian and Xu Cao
19. Studying the Functions of TGF-β Signaling in the Ovary
Chao Yu, Jian-Jie Zhou and Heng-Yu Fan
20. Quantitative Real-Time PCR Analysis of MicroRNAs and Their Precursors Regulated by TGF-b Signaling
Hara Kang and Akiko Hata
21. TGF-β Regulated microRNAs and Their Function in Cancer Biology
Pengyuan Yang, Yun Zhang, Geoffrey J. Markowitz, Xing Guo and Xiao-Fan Wang
22. Epigenomic Regulation Of Smad1 Signaling During Cellular Senescence Induced by Ras Activation
Atsushi Kaneda, AyaNonaka, Takanori Fujita, Ryota Yamanaka, Mai Fujimoto, Kohei Miyazono and Hiroyuki Aburatani
23. The Role of Ubiquitination to Determine Non-Smad Signaling Responses
Shyam Kumar Gudey and Marene Landström
24. Genome-wide RNAi Screening to Dissect the TGF-b Signal Transduction Pathway
Xiaochu Chen and LanXu
25. Measuring TGF-β Ligand Dynamics in Culture Medium
Zipei Feng and Xuedong Liu
