Part I. Isolation of T Cells from the Tissues
1. Isolation of T Cells from the Skin
Beate Lorenz and Esther von Stebut
2. Isolation of Leukocytes from Mouse Central Nervous System
Iva Lelios and Melanie Greter
3. Isolation of T Cells from the Gut
Sonja Reißig, Christopher Hackenbruch, and Nadine Hövelmeyer
4. T Cell Isolation from Mouse Kidneys
Isis Ludwig-Portugall and Christian Kurts
Part II. Analysis of T Cell Function and Phenotypes
5. Intracellular Staining for Cytokines and Transcription Factors
Florian Mair and Vinko Tosevski
6. Surface Capture Staining
Vinko Tosevski and Florian Mair
7. Mass Cytometry Analysis of Human T Cell Phenotype and Function
Evan W. Newell and Lai Li Yun
8. Detection of NF-κB Pathway Activation in T Helper Cells
Oliver Gorka, Stefan Wanninger, and Jürgen Ruland
9. Assessing the Suppressive Activity of Foxp3+ Regulatory T Cells
Christian Thomas Mayer and Tim Sparwasser
10. In vitro Generation of Microbe Specific Human Th17 Cells
Julia M. Braun and Christina E. Zielinski
11. In vitro Polarization of T-Helper Cells
Julie Rumble and Benjamin Segal
Part III. In vivo Models of T Cell Function
12. A Method for the Generation of TCR Retrogenic Mice
Elisa Kieback, Ellen Hilgenberg, and Simon Fillatreau
13. Mouse Models of Allergic Airway Disease
Helen Meyer-Martin, Sebastian Reuter, and Christian Taube
14. Induction of Colitis in Mice (T-Cell Transfer Model)
Benno Weigmann
15. Manipulation of T Cell Function Conditional Gene Targeting in T Cells
Anna Śledzińska, Lynsey Fairbairn, and Thorsten Buch
16. Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes
C.T. Wohn, S. Pantelyushin, J.L. Ober-Blöbaum, and B.E. Clausen
17. Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells
Rhoanne C. McPherson, Helen E. Cambrook, Richard A. O'Connor, and Stephen M. Anderton
18. Experimental Mouse Models of T Cell-Dependent Inflammatory Bowel Disease
George X. Song-Zhao and Kevin J. Maloy
19. Analysis of Chromosomal Aberrations in Murine HCC
Kristian Unger and Mathias Heikenwälder
