Section I Cell Cycle and Cellular Dynamics I.1 Novel Approaches for Cell Cycle Analysis in BY-2 Olga A. Koroleva, Gethin R. Roberts, Matthew L. Tomlinson, and John H. Doonan 1 Introduction 2 A Modified alc-Inducible System for Transgene Expression in BY-2 3 Use of the alc System to Define the Time of Action of Induced Protein 4 An Example of Functional Analysis Using the AlcR-GR Gene Switch 5 Transient Protein Expression 6 Emerging Techniques 7 Protocols 8 Composition of Media References I.2 Dynamics and Structure of the Preprophase Band and the Phragmoplast Danny Geelen and Dirk Inzé 1 Introduction 2 The Preprophase Band (PPB) 3 The Phragmoplast 4 Future Perspectives References I.3 Formation of Cortical Microtubules in a Cell-Free System Prepared from Plasma Membrane Ghosts and a Cytosolic Extract of BY-2 Cells Takashi Murata and Mitsuyasu Hasebe 1 Introduction 2 Microtubules Nucleate as Branches on Existing Microtubules in the Cortical Arrays of Plant Cells 3 Analysis of the Molecular Mechanisms of Microtubule Nucleation in a BY-2 Cell-Free System 4 Future Perspectives References I.4 Chromosome Dynamics in Tobacco BY-2 Cultured Cells Sachihiro Matsunaga, Nobuko Ohmido, and Kiichi Fukui 1 Introduction 2 Dynamic Analysis of Condensin Complexes 3 Dynamic Analysis of Heterochromatic Protein 1 4 Dynamic Analysis of Aurora Kinases 5 Conclusion and Perspectives References I.5 Ion Channels Meet Cell Cycle Control Rainer Hedrich and Dirk Becker 1 Introduction 2 Cell Elongation 3 Cell Cycle 4 Discussion References I.6 The NACK-PQR MAP Kinase Cascade Controls Plant Cytokinesis MichikoSasabe, Yuji Takahashi, Takashi Soyano, Hirokazu Tanaka, Ken Kousetsu, Takamasa Suzuki, and Yasunori Machida 1 Introduction 2 Plant Cytokinesis 3 The MAPK Cascade Involved in Cytokinesis 4 Regulation of the NACK-PQR Pathway in the Cell Cycle Machinery 5 Downstream Factors of the NACK-PQR Pathway 6 Future Perspectives References Section II Physiological and Developmental Aspects II.1 Characterization of a Cell Division Factor from Auxin-Autotrophic 2B-13 Cells Derived from the Tobacco BY-2 Cell Line Takashi Shimizu, Kentaro Eguchi, Ikuo Nishida, Kris Laukens, Erwin Witters, and Toshiyuki Nagata 1 Introduction 2 Characterization of Cell Division-Inducing Activity in the Culture Filtrates of 2B-13 Cells 3 Purification of the CDF 4 Searches for CDFs in the Culture Filtrates of Tobacco BY-2 Cells 5 Concluding Remarks and Future Perspectives 6 Protocols References II.2 The BY-2 Cell Line as a Tool to Study Auxin Transport Jan Petrásek and Eva Zazímalová 1 Introduction 2 Present State of the Art of Cell-to-Cell Transport of Auxins 3 Auxin Transport Studies in Planta 4 Auxin Transport Studies in Simplified Models 5 Concluding Remarks and Future Prospects References II.3 Tobacco BY-2 Cells as a Model for Differentiation in Heterotrophic Plant Cells Yutaka Miyazawa and Atsushi Sakai 1 Introduction 2 Hormonal Factors Affecting Starch-Storing Cell Differentiation in BY-2 Cells 3 Regulations of Gene Expressions Required for Differentiation and Dedifferentiation of Starch-Storing Cells 4 Conclusions and Perspectives References Section III Intracellular Traffic III.1 Imaging the Early Secretory Pathway in BY-2 Cells David G. Robinson