Part I. Introduction
1. Transgenic Cotton: From Biotransformation Methods to Agricultural Application
Baohong Zhang
2. Genetically Modified Cotton in India and Detection Strategies
Gurinder Jit Randhawa and Rashmi Chhabra
Part II. Transformation
3. Agrobacterium-Mediated Transformation of Cotton
Baohong Zhan
4. Biolistic Transformation of Cotton Zygotic Embryo Meristem
Rajah Rajasekaran
5. Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures
Rajah Rajasekaran
6. Cotton Transformation via Pollen Tube Pathway
Baohong Zhang and Qinglian Wang
7. Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.)
Muhammad Arshad, Yusuf Zafar, and Shaheen Asad
Part III. Detection
8. Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome
Jun Zhang and Yan Hong
9. Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR
Chengxin Yi and Yan Hong
10. Development of Enzyme-Linked Immunosorbant (ELISA) Assay for the Detection of Bt Protein in Transgenic Cotton
Abhishek Ojha, Arvind Kumar, and Suchitra Kamle
11. DNA-Based Diagnostics for Genetically Modified Cotton: Decaplex PCR Assay to Differentiate MON531 and MON15985 Bt Cotton Events
Gurinder Jit Randhawa, Monika Singh, and Rashmi Chhabra
12. A Simple and Rapid Method for Determining Transgenic Cotton Plants
Baohong Zhang, Hongmei Wang, Fang Liu, and Qinglian Wang
13. An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants
Min Wang, Qinglian Wang, and Baohong Zhang
14. Inheritance of Transgenes in Transgenic Bt Lines Resistance to Helicoerpa armigera in Upland Cotton
Baolong Zhang, Wangzhen Guo, and Tianzhen Zhang
Part IV. Application
15. Agrobacterium rhizogenes-Induced Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics
Hee Jin Kim
16. Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation
Baohong Zhang, Min Wang, Xin Zhang, Chengqi Li, and Qinglian Wang
17. Development of Transgenic CryIA(c)+GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover
Zhi Liu, Zhen Zhu, and Tianzhen Zhang
18. Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition
Zhiying Ma, Jianfeng Liu, and Xingfen Wang
19. Genetic Transformation of Cotton with a Harpin-Encoding Gene hpaXoo Confers an Enhanced Defense Response Against Verticillium dahliae Kleb
Weiguo Miao and Jingsheng Wang
20. Development of Insect-Resistant Transgenic Cotton with Chimeric TVip3A* Accumulating in Chloroplasts
Jiahe Wu and Yingchuan Tian
Part V. Risk Assessment
21. Determining Gene Flow in Transgenic Cotton
Xiaoping Pan