1. Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible
Hidetoshi Mori and Robert D. Cardiff
2. Laser Capture Microdissection as a Tool to Study Tumor Stroma
Nicholas R. Bertos and Morag Park
3. Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging
Lian Willetts, David Bond, Konstantin Stoletov, and John D. Lewis
4. Studies on the Tumor Vasculature and Coagulant Microenvironment
Esterina D'Asti, Brian Meehan, and Janusz Rak
5. A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment
José A. Jiménez-Torres, David J. Beebe, and Kyung E. Sung
6. Measuring Vascular Permeability In Vivo
Eelco F.J. Meijer, James W. Baish, Timothy P. Padera, and Dai Fukumura
7. Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL)
Pardeep Heir and Michael Ohh
8. Analyzing the Tumor Microenvironment by Flow Cytometry
Yoon Kow Young, Alicia M. Bolt, Ryuhjin Ahn, and Koren K. Mann
9. Detecting Secreted Analytes from Immune Cells: An Overview of Technologies
Kelly A. Pike, Caitlyn Hui, and Connie M. Krawczyk
10. Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-Parameter Flow Cytometry-Based Sorting
Camilla Salvagno and Karin E. de Visser
11. Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes
Joanne A. Hammill, Arya Afsahi, Jonathan L. Bramson, and Christopher W. Helsen
12. Methods to Evaluate the Anti-Tumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies
Bertrand Allard, David Allard, and John Stagg
13. Isolation and Characterization of Low Vs. High-Density Neutrophils in Cancer
Jitka Y. Sagiv, Sandra Voels, and Zvi Granot
14. Analysis of Extracellular Vesicles in the Tumor Microenvironment
Khalid Al-Nedawi and Jolene Read
15. Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disc Confocal Microscopy
Liane Babes and Paul Kubes
16. Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice
Victor Naumenko, Craig Jenne, and Douglas J. Mahoney
17. Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer
Chirayu Chokshi, Manvir Dhillon, Nicole McFarlane, Chitra Venugopal, and Sheila K. Singh
18. Modeling Breast Tumor Development with a Humanized Mouse Model
Lisa M. Arendt
19. CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice
Yojiro Yamanaka
20. Metabolomics Analyses of Cancer Cells in Controlled Microenvironments
Simon-Pierre Gravel, Daina Avizonis, and Julie St-Pierre
21. Analysis of the Tumor Microenvironment Transcriptome Via NanoString mRNA and miRNA Expression Profiling
Marie-Noël M'Boutchou and Léon C. van Kempen
22. RNA-Seq as a Tool to Study the Tumor Microenvironment
Pudchalaluck Panichnantakul, Mathieu Bourgey, Alexandre Montpetit, Guillaume Bourque, and Yasser Riazalhosseini
23. Sample Preparation for Mass Spectrometry Analysis of Protein-Protein Interactions in Cancer Cell Lines and Tissues
Alice B
