Part I: Background
1. ER to Golgi Dependent Protein Secretion: The Conventional Pathway
Corrado Viotti
2. Unconventional Protein Secretion in Animal Cells Fanny Ng and Bor Luen Tang
3. Unconventional Protein Secretion in Plants
Destiny J. Davis, Byung-Ho Kang, Angelo S. Heringer, Thomas E. Wilkop, and Georgia Drakakaki
Part II: UPS Contemplates Multidisciplinary Approaches for Plants
4. Chemical Secretory Pathway Modulation in Plant Protoplasts
Francesca De Marchis, Andrea Pompa, and Michele Bellucci
5. From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYGR) Model in Arabidopsis
Haiyan Zhang and Jinjin Li
6. Following the Time-Course of Post-Pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations
Darya Safavian, Jennifer Doucet, and Daphne R. Goring
Part III: UPS Contemplates Multidisciplinary Approaches for Animals and Fungi
7. Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Margarida D. Amaral, Carlos M. Farinha, Paulo Matos, and Hugo M. Botelho
8. Quantification of a Non-Conventional Protein Secretion: The Low Molecular Weight FGF-2 Example
Tania Arcondéguy, Christian Touriol, and Eric Lacazette
9. Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion
Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer
10. A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis Janpeter Stock, Marius Terfrüchte, and Kerstin Schipper
11. Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And Quantification
Marcos Vinicios Salles Dias, Vilma Regina Martins, and Glaucia Noeli Maroso Hajj 12. Analysis of Yeast Extracellular Vesicles
Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasés, Kildare Miranda, and Leonardo Nimrichter
13. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods
Lorna MacLean, Helen Price, and Peter O'Toole
14. Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40
Olivier Reynard and Mathieu Mateo
15. Role and Characterization of synuclein-γ Unconventional Protein Secretion in Cancer Cells
Caiyun Liu, Like Qu, and Chengchao Shou
Part IV: Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)
16. Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF)
Pavel Gromov and Irina Gromova
17. Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevine
Bertrand Delaunois, Fabienne Baillieul, Christophe Clément, Philippe Jeandet, and Sylvain Cordelier
18. Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D2O) Density Cushions
Christopher Stanly, Immacolata Fiume, Giovambattista Capasso, and Gabriella Pocsfalvi
