PART I VACCINES: INTRODUCTION
1. Challenges for Vaccinologists in the First Half of the Twenty First Century
Sunil Thomas, Ann Abraham, Patrick J. Callaghan, and Rino Rappuoli
2. Principles in Immunology for the Design and Development of Vaccines
Claudius U. Meyer and Fred Zepp
3. Revisiting the Principles of Designing a Vaccine
Shubhranshu Zutshi, Sunil Kumar, Prashant Chauhan, Bhaskar Saha
4. Status of COVID-19 Pandemic before the Administration of Vaccine
Sunil Thomas
PART II TRENDS IN VACCINOLOGY
5. mRNA Vaccines to Protect against Diseases
Sunil Thomas and Ann Abraham
6. Artificial Intelligence in Vaccine and Drug Design
Sunil Thomas, Ann Abraham, Jeremy Baldwin, Sakshi Piplani, and Nikolai Petrovsky
PART III VACCINES FOR HUMAN VIRAL DISEASES
7. Vaccines Targeting Numerous Coronavirus Antigens, Ensuring Broader Global Population Coverage: Multi-Epitope and Multi-Patch Vaccines
Sukrit Srivastava, Spyros D. Chatziefthymiou, Michael Kolbe
8. Use of Micro-computed Tomography to Visualize and Quantify COVID-19 Vaccine Efficiency in Free-breathing Hamsters
Laura Seldeslachts, Christopher Cawthorne, Suzanne F. Kaptein, Robbert Boudewijns, Hendrik Jan Thibaut, Lorena Sanchez Felipe, Sapna Sharma, Georg Schramm, Birgit Weynand, Kai Dallmeier and Greetje Vande Velde
9. Design of Replication-Competent VSV- and Ervebo-vectored Vaccines against SARS-CoV-2
Qixing Liu, Zhe Ding, Jiaming Lan, and Gary Wong
10. CRISPR Engineering of Bacteriophage T4 to Design Vaccines against SARS-CoV-2 and Emerging Pathogens
Jingen Zhu, Neeti Ananthaswamy, Swati Jain, Himanshu Batra, Wei-Chun Tang, and Venigalla B. Rao
11. Techniques for Developing and Assessing Immune Responses Induced by Synthetic DNA Vaccines for Emerging Infectious Diseases
Ziyang Xu, Michelle Ho, Devivasha Bordoloi, Sagar Kudchodkar, Makan Khoshnejad, Leila Giron, Faraz Zaidi, Moonsup Jeong, Christine C. Roberts, Young K. Park, Joel Maslow, Mohamed Abdel-Mohsen, and Kar Muthumani
12. Towards Determining the Epitopes of the Structural proteins of SARS-CoV-2
Sunil Thomas
13. Development, production and characterization of hepatitis B subviral envelope particles as a third-generation vaccine
Juan Manuel Battagliotti, Diego Fontana, Marina Etcheverrigaray, Ricardo Kratje, and Claudio Prieto
14. Generation of CpG-Recoded Zika Virus Vaccine Candidates
Ivan Trus, Daniel Udenze, Uladzimir Karniychuk
PART IV VACCINES FOR HUMAN BACTERIAL DISEASES
15. Salmonella Uptake into Gut-Associated Lymphoid Tissues: Implications for Targeted Mucosal Vaccine Design and Delivery
Angelene F. Richards, Fernando J. Torres-Velez, and Nicholas J. Mantis
16. Development of Human Recombinant Leptospirosis Vaccines
Natasha Rodrigues de Oliveira, Thaís Larré Oliveira, Sérgio Jorge, and Odir Antônio Dellagostin
17. Induction of T-cell Responses by Vaccination of a Streptococcus pneumoniae Whole Cell Vaccine
Emily M. Roy, Fan Zhang, Richard Malley, and Ying-Jie Lu
18. Development of a Bacterial Nanoparticle Vaccine against Escherichia coli Melibea Berzosa, Yadira Pastor, Carlos Gamazo, and Juan Manuel Irache
19. Construction of Novel Live Genetically Modified BCG Vaccine Candidates using Recombineering Tools
Mario Alberto Flores-Valdez and Michel de Jesús Aceves-Sánchez
20. An Update on Tuberculosis Vaccines
Radha Gopalaswamy and Selvakuma