VEGF Signaling

1. Opposing Roles of Vascular Endothelial Growth Factor C in Metastatic Dissemination and Resistance to Radio/Chemotherapy: Discussion of Mechanisms and Therapeutic Strategies

Christopher Montemagno, Frédéric Luciano, and Gilles Pagès

2. Identification of VEGF Isoforms in Mouse, Rat, and Zebrafish Using RT-qPCR

Patricia de Winter

3. Multi-Parameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells

4. Absolute Quantification of Plasma Membrane Receptors via Quantitative Flow Cytometry

Yingye Fang, Manasi Malik, Sarah K. England, and P.I. Imoukhuede

5. Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays

Ye Zeng, Xiaoqiang Du, Wenli Jiang, and Yan Qiu

6. SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling

Chiara Testini

7. Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells

William R. Critchley, Gareth W.F. Fearnley, Izma Abdul-Zani, Carmen Molina-Paris, Claus Bendtsen, Ian C. Zachary, Michael A. Harrison, and Sreenivasan Ponnambalam

8. Co-Immunoprecipitation Assays

Ian M. Evans and Ketevan Paliashvili

9. Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling

James A.G.E. Taylor, Christopher J. Benwell, and Stephen D. Robinson

10. RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens

Tiziana Annese, Roberto Tamma, and Domenico Ribatti

11. Global MicroRNA Profiling of Vascular Endothelial Cells

Eloi Schmauch, Anna-Liisa Levonen, and Suvi Linna-Kuosmanen

12. Endothelial Cell Tube Formation Assay: An In Vitro Model for Angiogenesis

Mary Kelley, Sara Fierstein, Laura Purkey, and Kathleen DeCicco-Skinner

13. Use of a Thin Layer Assay for Assessing the Angiogenic Potential of Endothelial Cells In Vitro

James A.E. Lane, Ashton Faulkner, Elizabeth J.T. Finding, Eleanor G. Lynam, and Caroline P.D. Wheeler-Jones

14. VEGF-A₁₆₅ -Induced Endothelial Cells Chemotactic Migration and Invasion Assays

Caroline Pellet-Many

15. Measuring Mitochondrial Calcium Fluxes in Cardiomyocyte upon Mechanical Stretch-Induced Hypertrophy

Daniela Ramaccini, Carlotta Giorgi, and Michelle L. Matter

16. Simultaneous Measurement of Endothelial Cell Proliferation and Cell Cycle Stage Using Flow Cytom