1. Approaches and Species in the History of Vertebrate Embryology
Nick Hopwood
2. Manipulating and Imaging the Early Xenopus laevis Embryo
Michael V. Danilchik
3. Manipulation of Gene Function in Xenopus laevis
Mizuho S. Mimoto and Jan L. Christian
4. Developmental Genetics in Xenopus tropicalis
Timoty J. Geach and Lyle B. Zimmerman
5. Embryological and Genetic Manipulation of Chick Development
Laura S. Gammill and Catherine E. Krull
6. Embryological Manipulations in Zebrafish
Yuhua Sun, Dorota Wloga, and Scott T. Dougan
7. Practical Approaches for Implementing Forward Genetic Strategies in Zebrafish
Sreelaja Nair and Francisco Pelegri
8. Essential Techniques for Introducing Medaka to a Zebrafish Laboratory - Toward the Combined Use of Medaka and Zebrafish for Further Genetic Dissection of the Function of the Vertebrate Genome
Sean R. Porazinski, Huijia Wang, and Makoto Furutani-Seiki
9. Ex-utero Culture and Imaging of Mouse Embryos
Anna Piliszek, Gloria S. Kwon, and Anna-Katerina Hadjantonakis
10. Detection of Gene Expression in Mouse Embryos and Tissue Sections
Edwina McGlinn and Jennifer H. Mansfield
11. Gene Targeting in the Mouse
Anne E. Griep, Manorama C. John, Sakae Ikeda, and Akihiro Ikeda
12. Creating a "Hopeful Monster" Mouse Forward Genetic Screens
Vanessa L. Horner and Tamara Caspary
13. Assisted Reproductive Technology in Nonhuman Primates
Tien-cheng Chang and Anthony W.S. Chan
14. Embryological Methods in Ascidians: the Villefranche-sur-Mer Protocols
Christian Sardet, Alex McDougall, Hitoyoshi Yasuo, Janet Chenevert, Gérard Pruliere, Rémi Dumollard, Clare Hudson, Celine Hebras, Ngan Le Nguyen and Alexandre Paix
15. Ciona Genetics
Michael T. Veeman, Shota Chiba and William C. Smith
16. Analyses of Gene Function in Amphioxus Embryos by Microinjection of mRNAs and Morpholino-oligonucleotides
Linda Z. Holland and Takayuki Onai
17. Reptile Embryology
Matthew K. Vickaryous and Katherine E. McLean
18. Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii
Marylin B. Renfree and Andrew J. Pask
19. Mutant Generation in Vertebrate Model Organisms by TILLING
Sylke Winkler, Nicola Gscheidel, and Michael Brand
20. Inducing High Rates of Targeted Mutagenesis in Zebrafish using Zinc Finger Nucleases (ZFNs)
Jasmine M. McCammon, Yannick Doyon, and Sharon L. Amacher
21. Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic Cell Nuclear Transfer
Zhongde Wang
22. Cloning Mice and ES Cells by Nuclear Transfer from Somatic Cells and Fully Differentiated Cells
Zhongde Wang
23. Keeping Two Animal Systems in One Lab: A Frog Plus Fish Case Study
Hazel Sive
24. Laboratory Guidelines for Animal Care
Marcelo Cuoto
