1. Introduction to Viral Vectors
James N. Warnock, Claire Daigre, and Mohamed Al-Rubeai
2. Introduction to Gene Therapy: A Clinical Aftermath
Patrice P. Denèfle
3. Host Cells and Cell Banking
Glyn N. Stacey and Otto-Wilhelm Merten
4. Overview of Current Scalable Methods for Purification of Viral Vectors
María Mercedes Segura, Amine Kamen, and Alain Garnier
5. Methods to Construct Recombinant Adenovirus Vectors
Miguel Chillon and Ramon Alemany
6. Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus
Edwige Dormond and Amine A. Kamen
7. Manufacturing of Retroviruses
Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana Amaral, Paula M. Alves, and Ana S. Coroadinha
8. Lentiviral Vectors
Marc Giry-Laterrière, Els Verhoeyen, and Patrick Salmon
9. Adeno-Associated Viruses
Mauro Mezzina and Otto-Wilhelm Merten
10. Manufacturing of Adeno-Associated Viruses, for Example: AAV2
Haifeng Chen
11. Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses
J. Fraser Wright and Olga Zelenaia
12. Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells
Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. Mähönen, and Seppo Ylä-Herttuala
13. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors
Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein
14. Manufacture of Measles Viruses
Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, and Mark J. Federspiel
15. In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model
Cecilia Frecha, Floriane Fusil, François-Loïc Cosset, and Els Verhoeyen
16. In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair)
Kolja Gelse and Holm Schneider
17. Ethical Consideration
Michael Fuchs
18. Clinical Trials of GMP Products in the Gene Therapy Field
Kathleen B. Bamford
