1. Basic Investigations in Saccharomyces cerevisiae
Brendan Curran and Virginia Bugeja
2. Isolation of Yeast Nucleic Acids
Michael Biss, Michelle Hanna and Wei Xiao
3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry
Ting Wei and Heping Dai
4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
R. Daniel Gietz
5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe
Johannes H. Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Markus Langen and Ursula Fleig
6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry
Changhui Deng and Andrew N. Krutchinsky
7. Yeast Two Hybrid Liquid Screening
Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz
8. Targeted Mutagenesis of a Specific Gene in Yeast
Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz
9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae
Kai Stefan Dimmer
10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy
Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie
11. Assays for Autophagy II: Mitochondrial Autophagy
Tomotake Kanki and Koji Okamoto
12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction
Danielle Hagstrom and Changle Ma
13. Yeast Survival and Growth Assays
Xin Xu, Amanda Lambrecht, and Wei Xiao
14. Spontaneous Mutagenesis Assay
Susan Blackwell, Michelle Hanna, and Wei Xiao
15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene
Bernard A. Kunz
16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe
Jun Li and Li-Lin Du
17. Replicative and Chronological Life Span Assays
Spike D. L. Postnikoff and Troy A. A. Harkness
18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae
Christopher Aa. Jackson and Michael C. Yu
19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae
Lindsay G. Ball and Wei Xiao
20. Ligand Engineering Using Yeast Surface Display
Benjamin J. Hackel
21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay
Kris Barreto and C. Ronald Geyer