Zelladhäsion auf Kollagen IV mit variabler Kopplung zu Polymersubstraten
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Zelladhäsion auf Kollagen IV mit variabler Kopplung zu Polymersubstraten

Zelladhäsion auf Kollagen IV mit variabler Kopplung zu Polymersubstraten


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About the Book

Masterarbeit aus dem Jahr 2008 im Fachbereich Medizin - Biomedizinische Technik, Note: A- very good, Politechnika Wroclaw, Sprache: Deutsch, Abstract: In der Diplomarbeit soll untersucht werden, wie sich Endothelzellen durch eine Regulierung ihrer Adhäsion an künstlichen Oberflächen verhalten. Dazu wurden als Modelloberflächen verschiedene Polymerschichten hergestellt, welche die Ankopplung von Molekülen der extrazellulären Matrix, wie z.B. Kollagen IV, an das Substrat steuern und damit auch die Adhäsion der Endothelzellen regulieren. In der Experimenten wurde untersucht, wie sich die Morphologie von Einzelzellen und sowie die entsprechenden intrazellulären und extrazellulären Adhäsionsstrukturen der Zellen entwickeln. Durch die unterschiedlichen physikochemischen Eigenschaften des Polymersubstrate wurde die Bindungsstärke von Kollagen IV zum Substrat variiert. Insbesondere soll die Umordnung des angebotenen Kollagen IV sowie die in diesem Prozess involvierten Zelladhäsionsrezeptoren charakterisiert werden. Mittels Fluoreszenzmarkierung sollte sowohl das Aktin-Zytoskelett, verschiedene Integrine, die fokalen Adhäsionspunkte (mittels Vinculin) sowie das extrazelluläre Kollagen IV am konfokalen Laser Scanning Microscop sichtbar gemacht und ausgewertet werden. Um die Ausbildung von kapillaren Netzwerken in einer künstlichen extrazellulären Matrix zu verbessern, soll die Adhäsion von Endothelzellen und die Reorganisation von vorbeschitetem Kollagen IV und diese in Abhängigkeit von lateralen Substratstrukturen untersucht werden.


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Product Details
  • ISBN-13: 9783640211869
  • Publisher: Grin Publishing
  • ISBN-10: 3640211863
  • Publisher Date: 15 Nov 2008


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